Cleaning decontamination columns general guidelines flow cells general.
Waters hplc system cleaning procedure.
Technical assistant 3 2 checking.
Flush the system using one of the cleaning mixtures as described in the cleaning solution table of the controlling contamination in uplc ms and hplc ms sytems guide 715001307.
Remove all solvent filters.
When working with any lc system setting it up and storing it is very important.
Objective to lay down the procedure for operation of hplc waters alliance 2695 separation module scope to ensure that the instrument performs satisfactory and gives accurate and reproducible data.
Controlling contamination in lc ms and hplc ms systems 715001307.
Disconnect the column and attach a union and flow directly to waste.
For information on other acquity uplc cleaning methods refer to the recommended cleaning mixtures for lc table in the con trolling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
Maintaining your hplc uhplc uplc systems.
Executive manager 4 0 accountability head of the department 5 0 procedure 5 1 for system cleaning.
2 0 scope this procedure is applicable for of all high performance liquid chromatography installed in quality control laboratory.
To clean the system.
When preparing a system to run your method it is always important to prime up the system to ensure you get fresh solvents in all of the required lines.
Use only hplc grade solvents and water filtered through 0 2.
Label bottles correctly with bottle content and filling date expiry date.
Ion exchange to remove any remaining ions.
No injections are needed with that.
It is necessary to restore the system flow path to a near new level of cleanliness note.
Responsibility quality control officer executive.
Water flush to clean acid from the system.
3 0 responsibility 3 1 doing.
To clean the system.
Place a b c and d seal wash and needle wash lines into a clean.
Reverse osmosis to remove most contaminants b.
10 nh4oh in water this removes peg and other.
Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems.
This sop shall be applicable for the hplc system waters alliances 2690 2695 separation modules.
Residues or contaminations may block filters or capillaries.
Water flush to clear customer s mobile phases from the system.
Water acetonitrile methanol ipa 1 1 1 1 with 2 5 fa.
The purification process should include all of the following steps.